🧫
AAV Serotype Comparison for LRRK2 Knockdown in PD
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-wiki-experiments-aav-sero
🧫 Experiment Protocol
Validation
Metadata
| experiment_type | validation |
| source | {'type': 'manual', 'source_name': 'wiki', 'extraction_date': '2026-04-16T01:00:16.900874Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['AAV'], 'diseases': ["Parkinson's Disease"]} |
| model_system | mouse |
| summary | # AAV Serotype Comparison for LRRK2 Knockdown in PD ## Background and Rationale Leucine-rich repeat kinase 2 (LRRK2) mutations are the most common genetic cause of Parkinson's disease (PD), with G2019 |
| replication_status | replicated |
| methodology_notes | Phase 1 (Week 0): Prepare AAV vectors encoding LRRK2 shRNA in serotypes AAV1, AAV2, AAV5, AAV9, and AAVrh10 (1×10^12 vg/ml). Acclimate 60 male C57BL/6 mice (8-10 weeks) for one week. Phase 2 (Week 1): |
| primary_outcome | Identification of optimal AAV serotype achieving >70% LRRK2 knockdown in substantia nigra dopaminergic neurons with significant motor function improvement and minimal inflammatory response at 6 months |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from wiki source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.900879'} |
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