🧫
Blood-Brain Barrier Aging and Neurodegeneration — From Leakage to Neuronal Loss
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-wiki-experiments-blood-br
🧫 Experiment Protocol
Validation
Metadata
| experiment_type | validation |
| source | {'type': 'manual', 'source_name': 'wiki', 'extraction_date': '2026-04-16T01:00:16.902933Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['ABCB1/ANGPT1/CAV1'], 'diseases': ['Neurodegeneration']} |
| model_system | cell_line |
| summary | # Blood-Brain Barrier Aging and Neurodegeneration — From Leakage to Neuronal Loss ## Background and Rationale Blood-brain barrier (BBB) integrity deteriorates with aging and is increasingly recognized |
| replication_status | replicated |
| methodology_notes | **Phase 1: Cell Culture Establishment (Days 1-7)** • Establish primary human brain microvascular endothelial cells (hBMECs) and co-culture with pericytes and astrocytes in transwell inserts • Seed hBM |
| primary_outcome | Quantification of BBB permeability changes in aged cell culture models and correlation with downstream neuronal death markers. |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from wiki source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.902940'} |
🌍 Provenance Graph
11 nodes, 44 edges
derives from (16)
...and 11 more
Linked Artifacts (2761)