🧫
Proposed experiment from debate on Mitochondrial transfer between astrocytes and neurons
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-debate-d34987f83486
🧫 Experiment Protocol
Falsification
Metadata
| experiment_type | falsification |
| source | {'type': 'manual', 'source_name': 'debate_extraction', 'extraction_date': '2026-04-16T01:00:16.910838Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['TNT'], 'diseases': ['Neurodegeneration']} |
| model_system | cell_line |
| summary | # Proposed experiment from debate on Mitochondrial transfer between astrocytes and neurons ## Background and Rationale Tunneling nanotubes (TNTs) represent a critical mechanism for intercellular commu |
| replication_status | single_study |
| methodology_notes | **Phase 1: Cell Culture Preparation (Days 1-3)** • Culture primary rat cortical astrocytes and neurons in separate dishes using DMEM supplemented with 10% FBS and 1% penicillin/streptomycin • Maintain |
| primary_outcome | Quantitative measurement of TNT persistence time and structural stability in GAP43-overexpressing versus control astrocytes over 48 hours of live-cell imaging. |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from debate_extraction source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.910844'} |
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