AMBRA1 Gene

Migration, Crosslink

📖

AMBRA1 Gene

active

wiki page Created: 2026-04-02T07:19:32 By: crosslink-migration Quality: 50% ✓ SciDEX ID: wiki-genes-ambra1

📖 Wiki Page

gene1882 wordssynced 2026-04-02

AMBRA1 Gene

<table class="infobox infobox-gene">
<tr>
<th class="infobox-header" colspan="2">ambra1</th>
</tr>
<tr>
<td class="label">Gene Symbol</td>
<td>AMBRA1</td>
</tr>
<tr>
<td class="label">Gene Name</td>
<td>Activating Molecule in Beclin 1-Regulated Autophagy 1</td>
</tr>
<tr>
<td class="label">Aliases</td>
<td>AMBRA1, KIAA1731</td>
</tr>
<tr>
<td class="label">Chromosomal Location</td>
<td>11p15.5</td>
</tr>
<tr>
<td class="label">NCBI Gene ID</td>
<td>55626</td>
</tr>
<tr>
<td class="label">UniProt ID</td>
<td>Q9C0A1</td>
</tr>
<tr>
<td class="label">Ensembl ID</td>
<td>ENSG00000164024</td>
</tr>
<tr>
<td class="label">Gene Type</td>
<td>Protein-coding</td>
</tr>
<tr>
<td class="label">Protein Family</td>
<td>WD40 repeat-containing proteins</td>
</tr>
<tr>
<td class="label">Approach</td>
<td>Model</td>
</tr>
<tr>
<td class="label">AAV-AMBRA1</td>
<td>AD mouse</td>
</tr>
<tr>
<td class="label">AAV-AMBRA1</td>
<td>PD model</td>
</tr>
<tr>
<td class="label">AMBRA1 overexpression</td>
<td>HD model</td>
</tr>
<tr>
<td class="label">Protein/Pathway</td>
<td>Interaction</td>
</tr>
<tr>
<td class="label">BECN1 (Beclin 1)</td>
<td>Direct binding</td>
</tr>
<tr>
<td class="label">VPS34</td>
<td>Complex formation</td>
</tr>
<tr>
<td class="label">ULK1</td>
<td>Phosphorylation</td>
</tr>
<tr>
<td class="label">ATG14L</td>
<td>Co-localization</td>
</tr>

...

AMBRA1 Gene

<table class="infobox infobox-gene">
<tr>
<th class="infobox-header" colspan="2">ambra1</th>
</tr>
<tr>
<td class="label">Gene Symbol</td>
<td>AMBRA1</td>
</tr>
<tr>
<td class="label">Gene Name</td>
<td>Activating Molecule in Beclin 1-Regulated Autophagy 1</td>
</tr>
<tr>
<td class="label">Aliases</td>
<td>AMBRA1, KIAA1731</td>
</tr>
<tr>
<td class="label">Chromosomal Location</td>
<td>11p15.5</td>
</tr>
<tr>
<td class="label">NCBI Gene ID</td>
<td>55626</td>
</tr>
<tr>
<td class="label">UniProt ID</td>
<td>Q9C0A1</td>
</tr>
<tr>
<td class="label">Ensembl ID</td>
<td>ENSG00000164024</td>
</tr>
<tr>
<td class="label">Gene Type</td>
<td>Protein-coding</td>
</tr>
<tr>
<td class="label">Protein Family</td>
<td>WD40 repeat-containing proteins</td>
</tr>
<tr>
<td class="label">Approach</td>
<td>Model</td>
</tr>
<tr>
<td class="label">AAV-AMBRA1</td>
<td>AD mouse</td>
</tr>
<tr>
<td class="label">AAV-AMBRA1</td>
<td>PD model</td>
</tr>
<tr>
<td class="label">AMBRA1 overexpression</td>
<td>HD model</td>
</tr>
<tr>
<td class="label">Protein/Pathway</td>
<td>Interaction</td>
</tr>
<tr>
<td class="label">BECN1 (Beclin 1)</td>
<td>Direct binding</td>
</tr>
<tr>
<td class="label">VPS34</td>
<td>Complex formation</td>
</tr>
<tr>
<td class="label">ULK1</td>
<td>Phosphorylation</td>
</tr>
<tr>
<td class="label">ATG14L</td>
<td>Co-localization</td>
</tr>
<tr>
<td class="label">Bcl-2</td>
<td>Binding</td>
</tr>
<tr>
<td class="label">DAPK1</td>
<td>Interaction</td>
</tr>
<tr>
<td class="label">Parkin</td>
<td>Mitochondrial quality</td>
</tr>
<tr>
<td class="label">PINK1</td>
<td>Mitophagy trigger</td>
</tr>
<tr>
<td class="label">TDP-43</td>
<td>ALS link</td>
</tr>
<tr>
<td class="label">LRRK2</td>
<td>PD link</td>
</tr>
<tr>
<td class="label">Associated Diseases</td>
<td><a href="/wiki/ad" style="color:#ef9a9a">AD</a>, <a href="/wiki/ali" style="color:#ef9a9a">ALI</a>, <a href="/wiki/als" style="color:#ef9a9a">ALS</a>, <a href="/wiki/age-related-macular-degeneration" style="color:#ef9a9a">Age-Related Macular Degeneration</a>, <a href="/wiki/aging" style="color:#ef9a9a">Aging</a></td>
</tr>
<tr>
<td class="label">KG Connections</td>
<td><a href="/atlas" style="color:#4fc3f7">745 edges</a></td>
</tr>
</table>

Pathway Diagram

Mermaid diagram (expand to render)

Overview

AMBRA1 (Activating Molecule in Beclin 1-Regulated Autophagy 1), also known as AMBRA1 or KIAA1731, encodes a critical positive regulator of autophagy that plays essential roles in cellular homeostasis, development, and disease pathogenesis[@cecconi2008][@fimia2012]. The AMBRA1 protein serves as a molecular bridge between autophagy initiation and execution, primarily through its interactions with the Beclin 1 complex and the core autophagy machinery.

The discovery of AMBRA1 represented a significant breakthrough in understanding how autophagy is regulated at the molecular level. Named for its ability to activate Beclin 1-dependent autophagy, AMBRA1 functions as a scaffold protein that brings together multiple components of the autophagy initiation machinery, thereby promoting the formation of autophagosomes—the double-membraned vesicles that encapsulate cellular components for degradation and recycling[@van2011].

Beyond its canonical role in autophagy, AMBRA1 has been implicated in a wide array of cellular processes including apoptosis regulation, cell cycle control, mitochondrial quality control through mitophagy, neurodevelopment, synaptic plasticity, and tumor suppression[@strappazzon2012]. Dysregulation of AMBRA1 has been strongly linked to neurodegenerative diseases including Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and various forms of cancer, making it an attractive therapeutic target[@antonioli2014].

Gene Information

Protein Structure and Molecular Function

Domain Architecture

The AMBRA1 protein (approximately 1,160 amino acids) contains several functional domains:

N-terminal domain: Contains multiple LC3-interacting regions (LIR motifs)

Central region: Harbors the Beclin 1-binding domain (BBD)

WD40 repeat domain (C-terminal): Forms a beta-propeller structure for protein-protein interactions

C-terminal domain: Contains additional regulatory sequences

Structural Features

Molecular weight: ~130 kDa
Quaternary structure: Forms homodimers and higher-order complexes
Post-translational modifications: Phosphorylation, ubiquitination, sumoylation

Molecular Functions

Autophagy Regulation

AMBRA1 serves as a master regulator of autophagy initiation through multiple mechanisms[@he2015][@mcnally2013]:

Beclin 1 complex assembly: AMBRA1 binds directly to Beclin 1 (BECN1) through its BBD, stabilizing the Beclin 1-PI3K complex (PI3KC3-C1)

PI3K activation: AMBRA1 facilitates the recruitment and activation of the class III PI3K VPS34, promoting PI3P production on nascent autophagosomes

ATG14L recruitment: AMBRA1 promotes the recruitment of ATG14L (also called BARKOR) to the ER-mitochondria contact sites (ERMES) where autophagosomes originate

ULK1 complex coordination: AMBRA1 interacts with the ULK1 complex (ULK1-ATG13-FIP200-ATG101), coordinating autophagy initiation with cellular energy and nutrient status

Apoptosis Regulation

Through interactions with Bcl-2 family proteins and caspases, AMBRA1 modulates apoptosis[@di2018]:

Competes with pro-apoptotic BH3-only proteins for Bcl-2 binding
Regulates the balance between autophagy and apoptosis
Functions in both cell death and survival pathways

Additional Functions

Mitochondrial quality control: Promotes PINK1-Parkin-dependent mitophagy
Cell cycle regulation: Interacts with DAPK1 and other cell cycle regulators
Synaptic function: Regulates synaptic vesicle trafficking and plasticity
Calcium homeostasis: Modulates ER calcium release and mitochondrial calcium uptake[@antonioli2017]

Expression Pattern

Tissue Distribution

AMBRA1 exhibits broad expression across tissues with highest levels in:

Brain: Particularly high in cerebral cortex, hippocampus, and cerebellum
Testis: High expression in spermatogenic cells
Liver: Moderate expression in hepatocytes
Heart: Present in cardiac myocytes
Kidney: Tubular epithelial cells

Cellular Distribution in the Brain

In the central nervous system:

[Neurons](/entities/neurons): High expression in pyramidal neurons, Purkinje cells, and hippocampal neurons
[Astrocytes](/entities/astrocytes): Moderate expression
[Microglia](/cell-types/microglia-neuroinflammation): Present in activated states
Oligodendrocytes: Lower expression

Regional Distribution

Cerebral [cortex](/brain-regions/cortex): High expression across all layers
[Hippocampus](/brain-regions/hippocampus): High in CA1-CA3 and dentate gyrus
[Cerebellum](/brain-regions/cerebellum): High in Purkinje cells
[Basal ganglia](/brain-regions/basal-ganglia): Present in striatum
Substantia nigra: Expression in dopaminergic neurons

Role in Neurodegenerative Diseases

Alzheimer's Disease

AMBRA1 dysfunction significantly contributes to AD pathogenesis through multiple mechanisms[@chang2019][@zhang2020]:

Autophagy Impairment

AMBRA1 levels are reduced in AD brains
Impaired AMBRA1-Beclin 1 interaction reduces autophagic flux
Accumulation of dysfunctional autophagosomes
Reduced clearance of [Aβ](/proteins/amyloid-beta) and [tau](/proteins/tau) aggregates

Amyloid-Beta Metabolism

[Aβ](/proteins/amyloid-beta) toxicity disrupts AMBRA1-Beclin 1 complex formation
Restoring AMBRA1 function enhances Aβ clearance
AMBRA1 deficiency increases Aβ accumulation
Autophagy-dependent and independent mechanisms

Tau Pathology

AMBRA1 regulates tau phosphorylation and aggregation
Autophagy enhancement reduces tau pathology
AMBRA1 deficiency exacerbates tau-induced neurodegeneration
Therapeutic potential of AMBRA1 activation

Synaptic Dysfunction

AMBRA1 in synaptic vesicle recycling
Impaired autophagy affects synaptic plasticity
Memory formation deficits
LTP impairment in model systems

Parkinson's Disease

AMBRA1 plays critical roles in PD through mitochondrial quality control and neuroprotection[@giampa2019][@yakhine-diop2019]:

Mitophagy Regulation

Essential for PINK1-Parkin-dependent mitophagy
AMBRA1 deficiency impairs mitochondrial clearance
Accumulates dysfunctional mitochondria in dopaminergic neurons
Contributes to energy failure and cell death

Alpha-Synuclein Pathology

[Alpha-synuclein](/proteins/alpha-synuclein) aggregation disrupts AMBRA1 function
AMBRA1 enhances α-synuclein clearance via autophagy
Reduced AMBRA1 promotes α-synuclein propagation
Links autophagy dysfunction to protein aggregation

LRRK2 Interactions

[LRRK2](/genes/lrrk2) mutations affect AMBRA1-mediated autophagy
LRRK2 kinase activity phosphorylates AMBRA1
Dysregulated AMBRA1 contributes to LRRK2 pathogenesis

Dopaminergic Neuron Survival

AMBRA1 protects substantia nigra neurons
Mitochondrial dysfunction in PD models
Therapeutic potential of AMBRA1 activation

Huntington's Disease

AMBRA1 is implicated in HD pathogenesis through mutant [huntingtin](/proteins/huntingtin-protein) interactions[@twitchell2017][@ma2019]:

Mutant Huntingtin Effects

Mutant huntingtin (mHtt) impairs AMBRA1 function
Disrupts AMBRA1-Beclin 1 interaction
Reduces autophagic clearance of mHtt aggregates
Exacerbates HD pathology

Therapeutic Potential

AMBRA1 overexpression improves mHtt clearance
Enhances motor function in models
Extends lifespan in animal models
Promising therapeutic target

Other Neurodegenerative Conditions

Amyotrophic Lateral Sclerosis (ALS)

TDP-43 pathology affects AMBRA1 function
Autophagy impairment in motor neurons
Mitochondrial dysfunction

Frontotemporal Dementia

Tau pathology links to AMBRA1 dysfunction
Autophagy defects in FTD models

Biological Functions and Cellular Processes

Autophagy Initiation

The canonical function of AMBRA1 in autophagy initiation involves[@yoo2018]:

Initiation complex formation: AMBRA1 bridges ULK1 and Beclin 1 complexes

VPS34 activation: Stimulates lipid kinase activity

Phagophore nucleation: Promotes PI3P production at isolation membranes

Expansion coordination: Recruits ATG proteins for autophagosome expansion

Apoptosis-Autophagy Crosstalk

AMBRA1 integrates signals from both pathways:

Competes with pro-apoptotic proteins for Bcl-2 binding
Promotes survival under stress conditions
Excessive stress shifts balance toward apoptosis

Neurodevelopment

During development[@fimia2012][@gonzalez2019]:

Essential for proper neural tube formation
Regulates neuronal proliferation and differentiation
Controls brain size through autophagy regulation
Knockout causes embryonic lethality

Synaptic Function

In mature neurons[@song2022]:

Regulates synaptic vesicle cycling
Important for presynaptic homeostasis
Controls postsynaptic plasticity
Memory formation and learning

Therapeutic Strategies

Targeting AMBRA1

Agonist Development

Small molecule activators: Enhancing AMBRA1-Beclin 1 interaction
Gene therapy: AAV-mediated AMBRA1 overexpression
Protein stabilization: Preventing AMBRA1 degradation

Challenges

Essential nature requires careful dosing
Blood-brain barrier penetration needed
Balancing autophagy enhancement with potential side effects

Preclinical Studies

Interacting Partners and Pathway Interactions

Research Tools and Models

Animal Models

Complete knockout: Embryonic lethal (E13.5-14.5)
Conditional knockouts: Brain-specific, neuron-specific
Transgenic overexpression: Various models
Humanized models: For drug testing

Cell Models

Primary neurons and astrocytes
iPSC-derived neural cells
Disease-specific cell lines
Organoid models

Pharmacological Tools

Activators: Naturally occurring (resveratrol,rapamycin indirectly)
Inhibitors: Various autophagy inhibitors
Detection: Antibodies for AMBRA1, LC3, p62

[Autophagy-Lysosomal Pathway](/mechanisms/autophagy-lysosomal-pathway) - Complete autophagy overview
[Mitophagy Pathway](/mechanisms/mitochondrial-dysfunction-pathway) - Mitochondrial clearance
[Beclin 1 Complex](/proteins/becn1-protein) - PI3K complex
[ULK1 Complex](/proteins/ulkt-protein) - Initiation complex
[Apoptosis Regulation](/mechanisms/apoptosis-pathway) - Cell death

[Alzheimer's Disease](/diseases/alzheimers-disease) - AD overview
[Parkinson's Disease](/diseases/parkinsons-disease) - PD overview
[Huntington's Disease](/diseases/huntingtons-disease) - HD overview
[Amyloid Beta](/proteins/amyloid-beta) - AD protein
[Tau](/proteins/tau) - AD protein
[Alpha-Synuclein](/proteins/alpha-synuclein) - PD protein
[Huntingtin Protein](/proteins/huntingtin-protein) - HD protein
[LRRK2](/genes/lrrk2) - PD gene

[BECN1](/proteins/becn1-protein) - Beclin 1
[VPS34](/proteins/vps34-protein) - PI3K class III
[ATG14L](/proteins/atk14l-protein) - Autophagy protein
[ULK1](/proteins/ulkt-protein) - Kinase complex

Current Research and Future Directions

Unmet Needs

Brain-penetrant AMBRA1 activators
Understanding cell-type-specific functions
Optimal delivery systems
Biomarker development

Emerging Areas

Gene therapy: AAV vectors for AMBRA1 delivery

Small molecule development: Direct activators

Combination therapies: With other autophagy modulators

Biomarkers: Patient selection and monitoring

External Links

[NCBI Gene - AMBRA1](https://www.ncbi.nlm.nih.gov/gene/55626)
[UniProt - Q9C0A1](https://www.uniprot.org/uniprot/Q9C0A1)
[Ensembl - ENSG00000164024](https://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000164024)
[GeneCards - AMBRA1](https://www.genecards.org/cgi-bin/carddisp.pl?gene=AMBRA1)

References

[Pickford F et al, Beclin 1 reduced expression in early Alzheimer disease (2008)](https://pubmed.ncbi.nlm.nih.gov/18599691/)

[Van Humbeeck C et al, The Beclin 1 interactome in neurodegeneration (2011)](https://pubmed.ncbi.nlm.nih.gov/21857024/)

[Twitchell B et al, Ambra1 haploinsufficiency in Huntington disease models (2017)](https://pubmed.ncbi.nlm.nih.gov/29106773/)

[Fimia GM et al, Ambra1 regulates autophagy and development of the nervous system (2012)](https://pubmed.ncbi.nlm.nih.gov/22522654/)

[Cecconi F and Levine B, The role of autophagy in mammalian development (2008)](https://pubmed.ncbi.nlm.nih.gov/18591655/)

[He C and Levine B, Beclin 1: an interaction hub for apoptosis, autophagy and endocytosis (2015)](https://pubmed.ncbi.nlm.nih.gov/25900965/)

[McNally KE et al, Ambra1 is a key regulator of autophagosome formation (2013)](https://pubmed.ncbi.nlm.nih.gov/23410032/)

[Antonioli M et al, Ambra1: a master regulator of autophagy and neurodegeneration (2014)](https://pubmed.ncbi.nlm.nih.gov/24813756/)

[Chang C et al, Ambra1 regulates amyloid-beta clearance in AD models (2019)](https://pubmed.ncbi.nlm.nih.gov/31571646/)

[Giampa C et al, Ambra1 expression in the brain and Parkinson disease (2019)](https://pubmed.ncbi.nlm.nih.gov/31026347/)

[Yakhine-Diop SM et al, Ambra1 deficiency contributes to mitochondrial dysfunction in PD (2019)](https://pubmed.ncbi.nlm.nih.gov/31365280/)

[Ma S et al, Ambra1 in the pathogenesis of Huntington disease (2019)](https://pubmed.ncbi.nlm.nih.gov/31237852/)

[Strappazzon F et al, Ambra1 in tumor growth (2012)](https://pubmed.ncbi.nlm.nih.gov/22575854/)

[Di Rita A et al, Ambra1 and BECN1: linking autophagy and apoptosis (2018)](https://pubmed.ncbi.nlm.nih.gov/29929475/)

[Antonioli M et al, Ambra1 regulates Ca2+ homeostasis in neurons (2017)](https://pubmed.ncbi.nlm.nih.gov/28082152/)

[Yoo SM and Jung YK, Molecular approach to understanding Ambra1 (2018)](https://pubmed.ncbi.nlm.nih.gov/29467518/)

[Zhang T et al, Ambra1 regulates tau pathology in AD models (2020)](https://pubmed.ncbi.nlm.nih.gov/32251429/)

[Song L et al, Ambra1 in synaptic plasticity and memory (2022)](https://pubmed.ncbi.nlm.nih.gov/35066253/)

[Gonzalez Y et al, Ambra1 in neurodevelopment (2019)](https://pubmed.ncbi.nlm.nih.gov/30628759/)

[Peyravian N et al, Ambra1 as a therapeutic target in neurodegeneration (2019)](https://pubmed.ncbi.nlm.nih.gov/30479204/)

[Marin M et al, Ambra1 in development and disease (2020)](https://pubmed.ncbi.nlm.nih.gov/32139462/)

[Thellmann S et al, Ambra1 knockdown enhances alpha-synuclein aggregation (2020)](https://pubmed.ncbi.nlm.nih.gov/32921047/)

Pathway Diagram

The following diagram shows the key molecular relationships involving AMBRA1 Gene discovered through SciDEX knowledge graph analysis:

Mermaid diagram (expand to render)

📖 View canonical wiki page →

Related Entities

AMBRA1

▸Metadataorigin_type: v1_polymorphic_backfill

slug	genes-ambra1
kg_node_id	AMBRA1
entity_type	gene
origin_type	v1_polymorphic_backfill
source_table	wiki_pages
wiki_page_id	wp-7b9d6df188f9
__merged_from	{'merged_at': '2026-05-13', 'unprefixed_id': 'genes-ambra1'}
_schema_version	1

📊 Evidence Profile Foundational

Evidence Balance

+0%

Certainty

100%

Debates

0

Incoming

214

Outgoing

36

0 supporting 0 contradicting 0 neutral

View full evidence profile →

Public annotations (0)Annotate on Hypothes.is →

No public annotations yet.

📗 Cite This Artifact

AMBRA1 Gene

AMBRA1 Gene

AMBRA1 Gene

Pathway Diagram

Overview

Gene Information

Protein Structure and Molecular Function

Domain Architecture

Structural Features

Molecular Functions

Autophagy Regulation

Apoptosis Regulation

Additional Functions

Expression Pattern

Tissue Distribution

Cellular Distribution in the Brain

Regional Distribution

Role in Neurodegenerative Diseases

Alzheimer's Disease

Autophagy Impairment

Amyloid-Beta Metabolism

Tau Pathology

Synaptic Dysfunction

Parkinson's Disease

Mitophagy Regulation

Alpha-Synuclein Pathology

LRRK2 Interactions

Dopaminergic Neuron Survival

Huntington's Disease

Mutant Huntingtin Effects

Therapeutic Potential

Other Neurodegenerative Conditions

Amyotrophic Lateral Sclerosis (ALS)

Frontotemporal Dementia

Biological Functions and Cellular Processes

Autophagy Initiation

Apoptosis-Autophagy Crosstalk

Neurodevelopment

Synaptic Function

Therapeutic Strategies

Targeting AMBRA1

Agonist Development

Challenges

Preclinical Studies

Interacting Partners and Pathway Interactions

Research Tools and Models

Animal Models

Cell Models

Pharmacological Tools

Cross-Linking and Related Pathways

Related Mechanisms

Related Diseases and Proteins

Related Genes

Current Research and Future Directions

Unmet Needs

Emerging Areas

See Also

External Links

References

Pathway Diagram

💬 Discussion