🧫
s:** - Compare uptake with/without magnetic particles using tight junction integrity markers - Test whether clustering occurs at BBB-relevant TfR expr
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-debate-b68856276b4a
🧫 Experiment Protocol
Falsification
Metadata
| experiment_type | falsification |
| source | {'type': 'manual', 'source_name': 'debate_extraction', 'extraction_date': '2026-04-16T01:00:16.910312Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['ABCB1/CAV1/CLDN5'], 'diseases': ['Neurodegeneration']} |
| model_system | cell_line |
| summary | # s:** - Compare uptake with/without magnetic particles using tight junction integrity markers - Test whether clustering occurs at BBB-relevant TfR expr ## Background and Rationale # Magnetic Particle |
| replication_status | single_study |
| methodology_notes | **Phase 1: Cell Culture Preparation (Days 1-7)** • Culture brain microvascular endothelial cells (hCMEC/D3 or bEnd.3) on Transwell inserts (0.4 μm pore, 24-well format) • Maintain cells in EBM-2 mediu |
| primary_outcome | Quantitative assessment of tight junction integrity using transendothelial electrical resistance (TEER) measurements and immunofluorescence analysis of junction proteins in brain endothelial cells tre |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from debate_extraction source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.910319'} |
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