🧫
Proposed experiment from debate on TDP-43 undergoes liquid-liquid phase separation that becomes pathological. Small
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-debate-7c7a8ed89fd3
🧫 Experiment Protocol
Falsification
Metadata
| experiment_type | falsification |
| source | {'type': 'manual', 'source_name': 'debate_extraction', 'extraction_date': '2026-04-16T01:00:16.909076Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['G3BP'], 'diseases': ['Neurodegeneration']} |
| model_system | cell_line |
| summary | # Proposed experiment from debate on TDP-43 undergoes liquid-liquid phase separation that becomes pathological. Small ## Background and Rationale This experiment tests the hypothesis that G3BP protein |
| replication_status | single_study |
| methodology_notes | **Phase 1: Cell Culture Preparation (Days 1-3)** • Maintain HEK293T, SH-SY5Y, and primary cortical neurons in appropriate media • Transfect cells with TDP-43 wildtype, A315T, and M337V constructs usin |
| primary_outcome | Quantification of insoluble TDP-43 aggregates using filter trap assays and biochemical fractionation in G3BP-deficient cells under non-stress granule inducing conditions compared to controls. |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from debate_extraction source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.909083'} |
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